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(Plasmid #67843)


Item Catalog # Description Quantity Price (USD)
Plasmid 67843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pET-30a
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5260
  • Total vector size (bp) 5772
  • Modifications to backbone
    modified MCS, pelB leader sequence was introduced, addittional in frame His-tag was introduced
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    antibody 1D8 light kappa chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The antibody 1D8 light kappa chain was derived from Tomlinson I+J phagemid library.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-scFv-T was a gift from Joanna Bereta (Addgene plasmid # 67843 ; ; RRID:Addgene_67843)
  • For your References section:

    A new expression vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson I+J phagemid libraries. Ossysek K, Uchanski T, Kulesza M, Bzowska M, Klaus T, Wos K, Madej M, Bereta J. Immunol Lett. 2015 Jul 25. pii: S0165-2478(15)30011-0. doi: 10.1016/j.imlet.2015.07.005. 10.1016/j.imlet.2015.07.005 PubMed 26219832