This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

hPDI1_18-508_WT_pFLAG-CMV1
(Plasmid #31382)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31382 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFLAG_CMV1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4732
  • Total vector size (bp) 6188
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPDI1
  • Alt name
    prolyl 4-hydroxylase, beta polypeptide
  • Alt name
    PDIA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1514
  • Entrez Gene
    P4HB (a.k.a. CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB)
  • Tag / Fusion Protein
    • FLAG M2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer FLAG CMV 2AS
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A bacterial expression vector for human PDI1 (hPDI1(18-508)pTrcHis-A) was a gift of Professor Colin Thorpe for U Delaware
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPDI1_18-508_WT_pFLAG-CMV1 was a gift from David Ron (Addgene plasmid # 31382 ; http://n2t.net/addgene:31382 ; RRID:Addgene_31382)
  • For your References section:

    Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin. Zito E, Melo EP, Yang Y, Wahlander Å, Neubert TA, Ron D. Mol Cell. 2010 Dec 10. 40(5):787-97. 10.1016/j.molcel.2010.11.010 PubMed 21145486