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hPDI1-18-508_C400S_C56S-pFLAG-CMV1
(Plasmid #31383)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31383 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDI1A
  • Alt name
    prolyl 4-hydroxylase, beta polypeptide
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1514
  • Mutation
    C-terminal cysteines of both active sites mutated to serine (C400S and C56S)
  • Entrez Gene
    P4HB (a.k.a. CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB)
  • Tag / Fusion Protein
    • FLAG M2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV_for
  • 3′ sequencing primer CMVR
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A bacterial expression vector for human PDI1 (hPDI1(18-508)pTrcHis-A) was a gift of Professor Colin Thorpe of U Delaware
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPDI1-18-508_C400S_C56S-pFLAG-CMV1 was a gift from David Ron (Addgene plasmid # 31383 ; http://n2t.net/addgene:31383 ; RRID:Addgene_31383)
  • For your References section:

    Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin. Zito E, Melo EP, Yang Y, Wahlander Å, Neubert TA, Ron D. Mol Cell. 2010 Dec 10. 40(5):787-97. 10.1016/j.molcel.2010.11.010 PubMed 21145486