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hPDI1-18-508_C400S-pFLAG-CMV1
(Plasmid #31384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31384 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 31384-D50 50 μg of DNA in Tris buffer $495
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DNA 31384-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFLAG_CMV1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4732
  • Total vector size (bp) 6188
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPDI1
  • Alt name
    prolyl 4-hydroxylase, beta polypeptide
  • Alt name
    PDIA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1514
  • Mutation
    C400S
  • Entrez Gene
    P4HB (a.k.a. CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB)
  • Tag / Fusion Protein
    • FLAG M1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer HGH-polyA reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPDI1-18-508_C400S-pFLAG-CMV1 was a gift from David Ron (Addgene plasmid # 31384 ; http://n2t.net/addgene:31384 ; RRID:Addgene_31384)

  • For your References section:

    Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin. Zito E, Melo EP, Yang Y, Wahlander Å, Neubert TA, Ron D. Mol Cell. 2010 Dec 10. 40(5):787-97. 10.1016/j.molcel.2010.11.010 PubMed 21145486
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