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One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.
Li S, Zhang A, Xue H, Li D, Liu Y
Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102559PB-SAMPiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
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102560PB-sgRNA(MS2)Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
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102563PB-SAM-DestPiggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
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