Purpose(Empty Backbone) Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102560||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3721
Vector typeMammalian Expression, CRISPR ; piggybac transposon
- Promoter U6
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer tttcttctataaagtaac
- 3′ sequencing primer gcattacacgtcttgagc (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-sgRNA(MS2) was a gift from Ying Liu (Addgene plasmid # 102560 ; http://n2t.net/addgene:102560 ; RRID:Addgene_102560)
For your References section:One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Li S, Zhang A, Xue H, Li D, Liu Y. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. 10.1016/j.omtn.2017.06.007 PubMed 28918057