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Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities.
Mehta S, Zhang Y, Roth RH, Zhang JF, Mo A, Tenner B, Huganir RL, Zhang J
Nat Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. doi: 10.1038/s41556-018-0200-6.
PubMed

Plasmids from Article

ID Plasmid Purpose  
118405pcDNA3.1(+)-ExRai-AKARcpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
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118406pcDNA3.1(+)-ExRai-AKAR(T/A)Negative-control mutant for ExRai-AKAR biosensor.
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118407pcDNA3.1(+)-ExRai-AKAR-NESCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
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118408pcDNA3.1(+)-ExRai-AKAR-KrasPlasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
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118409pcDNA3-ExRai-CKARcpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.
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118410pcDNA3-ExRai-CKAR(T/A)Negative-control mutant for ExRai-CKAR biosensor.
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118462pcDNA3-ExRai-AktARcpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.
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118463pcDNA3-ExRai-AktAR(T/A)Negative-control mutant for ExRai-AktAR biosensor.
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118464pcDNA3.1(+)-sapphireAKARcp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.
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118465pcDNA3.1(+)-sapphireAKAR(T/A)Negative-control mutant for sapphireAKAR biosensor.
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118466pcDNA3-Lyn-sapphireAKARcp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.
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118467pcDNA3.1(+)-sapphireAKAR-NLScp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.
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118468pcDNA3-sapphireCKARcp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.
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118469pcDNA3-sapphireCKAR(T/A)Negative-control mutant for sapphireCKAR biosensor.
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118470pcDNA3.1(+)-blueAKARcpBFP-based single-color biosensor for monitoring Protein Kinase A activity.
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118471pcDNA3.1(+)-blueAKAR(T/A)Negative-control mutant for blueAKAR biosensor.
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118472pcDNA3-blueCKARcpBFP-based single-color biosensor for monitoring Protein Kinase C activity.
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118473pcDNA3-blueCKAR(T/A)Negative-control mutant for blueCKAR biosensor.
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118474pcDNA3-RAB-EKARevddRFP-based single-color biosensor for monitoring ERK activity.
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118475pcDNA3-RAB-EKARev(T/A)Negative-control mutant for RAB-EKARev biosensor.
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118476pcDNA3-RAB-EKARev-NLSddRFP-based single-color ERK biosensor targeted to the nucleus.
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118477pcDNA3-Lyn-RAB-EKARevddRFP-based single-color ERK biosensor targeted to the plasma membrane.
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118478pcDNA3-RAB-AKARevddRFP-based single-color biosensor for monitoring Protein Kinase A activity.
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118479pcDNA3-RAB-AKARev(T/A)Negative-control mutant for RAB-AKARev biosensor.
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118480pcDNA3-RAB-ICUEddRFP-based single-color biosensor for monitoring cAMP dynamics.
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118482pcDNA3.1(+)-PKI-GFPGFP-tagged Protein Kinase A inhibitor peptide.
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