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Addgene

pJY-RpABE
(Plasmid #112872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKI1.1R
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RPS5A promoter
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1664

Gene/Insert 2

  • Gene/Insert name
    ABE7.10
  • Species
    engineered
  • Insert Size (bp)
    5328

Gene/Insert 3

  • Gene/Insert name
    U6-sgRNA cassette
  • Insert Size (bp)
    548

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A binary vector for plant adenine base editing through sgRNA cloning using AarI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJY-RpABE was a gift from Jin-Soo Kim & Jae-Young Yun (Addgene plasmid # 112872 ; http://n2t.net/addgene:112872 ; RRID:Addgene_112872)

  • For your References section:

    Precision genome engineering through adenine base editing in plants. Kang BC, Yun JY, Kim ST, Shin Y, Ryu J, Choi M, Woo JW, Kim JS. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. 10.1038/s41477-018-0178-x [pii] PubMed 29867128
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