168,499 results

1. RFP-HA-DAT

   Plasmid

   #90265

   Purpose

   Expresses RFP-HA-DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Expression

   Mammalian

   Mutation

   inserted HA tag (see YFP-HA-DAT)

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CapChR2_pmScarlet-N1

   Plasmid

   #188032

   Purpose

   Expresses CapChR2-mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3008

   Plasmid

   #144484

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RP156 pUAST EGFP:Gr21a

   Plasmid

   #59524

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Gr21 (Gr21a Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1-a and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGFP-N1_CBP

   Plasmid

   #221903

   Purpose

   Transient expression of GFP-tagged CBP in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBP (CREBBP Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-DeAct-GS1

   Plasmid

   #89445

   Purpose

   Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

    Plasmid

    #124362

    Purpose

    Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-aptNFkB#5
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIQ

    Plasmid

    #216238

    Purpose

    All-in One IQ transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNeonGreen-KDEL

    Plasmid

    #226568

    Purpose

    Encodes mNeonGreen with KDEL ER lumenal target sequence

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    KDEL (ER lumenal target sequence)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORTMAGE-4

    Plasmid

    #72679

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PH-PLCd1-mNGx3

    Plasmid

    #202753

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-L1-ESYN-L4

    Plasmid

    #32580

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    Enhanced Synapsin Promoter
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVTNT-T7-KPNA6

    Plasmid

    #26682

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 6 (KPNA6 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

    Plasmid

    #233628

    Purpose

    Expression of bicistronic reporter with TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSC5

    Plasmid

    #104779

    Purpose

    pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 5KKP (PUS7)

    Plasmid

    #101878

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PUS7 (PUS7 Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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