166,481 results

1. Mini-human AsCpf1-based human genome-wide knockout library

   Pooled Library

   #130630

   Purpose

   Expresses crRNAs for genome-wide knockout pooled library screening

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pNMT

   Plasmid

   #42578

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   N-myristoyl tranferase (NMT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations T690C and T942C

   Promoter

   T7

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

3. human p53-(82-393)

   Plasmid

   #24867

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 82-393

   Promoter

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-TRE3G-N-cG-CMV-P

   Plasmid

   #192886

   Purpose

   Tet inducible Nanoluc copGFP expression

   Depositor

   Kazuhiro Oka

   Insert

   Nanoluc-P2A-copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. LeGO-G2

   Plasmid

   #25917

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-E-cadherin promoter

   Plasmid

   #61798

   Purpose

   Luciferase reporter containing the mouse E-cadherin promoter

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDESTmycDICER

   Plasmid

   #19873

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   dicer 1, ribonuclease type III (DICER1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTALYM3-Telo1

   Plasmid

   #47883

   Purpose

   TALE-mClover against telomere sequence

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

   Insert

   TALE-mClover
   

   Use

   TALEN

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid

    #177316

    Purpose

    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

    Depositor

    Hirokazu Hirai

    Article

    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

    Insert

    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-mIFITM3

    Plasmid

    #58389

    Purpose

    Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. cycLuc_TEVS

    Plasmid

    #119207

    Purpose

    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with TEVs cleavage site
    

    Use

    Luciferase

    Tags

    6xHis and AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_PGK_SNIPR_CD8alpha variant 2

    Plasmid

    #188362

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_SFFv_Her2-BFP_RHL027

    Plasmid

    #164823

    Purpose

    Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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