Stat1 alpha Y701F Flag pRc/CMV
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8702||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSTAT1 alpha Y701F
Alt nameSTAT1 alpha
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneSTAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb). Author's Map shows the wild-type version of this plasmid (without the Y701F mutation).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Stat1 alpha Y701F Flag pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8702 ; http://n2t.net/addgene:8702 ; RRID:Addgene_8702)
For your References section:Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen Z, Zhong Z, Darnell JE Jr. Cell 1995 Jul 28;82(2):241-50. 10.1016/0092-8674(95)90311-9 PubMed 7543024
Map uploaded by the depositor.