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Stat1 beta Flag pRc/CMV
(Plasmid #8704)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8704 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT1 beta
  • Alt name
    STAT1b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • GenBank ID
    M97936
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
  • Tag / Fusion Protein
    • Flag Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat1 beta Flag pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8704 ; http://n2t.net/addgene:8704 ; RRID:Addgene_8704)
  • For your References section:

    Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr. Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9. 10.1073/pnas.89.16.7836 PubMed 1502203