Stat1 beta Flag pRc/CMV
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8704||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSTAT1 beta
SpeciesH. sapiens (human)
Insert Size (bp)2200
Entrez GeneSTAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
/ Fusion Protein
- Flag Tag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Stat1 beta Flag pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8704 ; http://n2t.net/addgene:8704 ; RRID:Addgene_8704)
For your References section:Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr. Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9. 10.1073/pnas.89.16.7836 PubMed 1502203