Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14875||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameB kinase activity reporter
/ Fusion Proteins
- CFP (N terminal on insert)
- YFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
BKAR was generated through substitution of a PKB substrate sequence, RKRDRLGTLGI, for the substrate sequence within pcDNA3-CKAR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BKAR was a gift from Alexandra Newton (Addgene plasmid # 14875 ; http://n2t.net/addgene:14875 ; RRID:Addgene_14875)
For your References section:Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC. J Biol Chem. 2005 Feb 18. 280(7):5581-7. 10.1074/jbc.M411534200 PubMed 15583002
Map uploaded by the depositor.