DKAR
(Plasmid #14879)

• Depositing Lab: Alexandra Newton
• Publication: Kunkel et al J Biol Chem. 2007 Mar 2. 282(9):6733-42.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: D kinase activity reporter
• Alt name: CFP-FHA2-peptide-YFP
• Tags / Fusion Proteins:
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

DKAR was generated through substitution of a PKD substrate sequence, LSRQLTAAVSE, for the substrate sequence within CKAR.