pTRE-TIGHT miR-1 (aka:pTRE-m1d)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14896||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBartel Lab
- Backbone size w/o insert (bp) 2600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)500
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site ClaI (unknown if destroyed)
- 5′ sequencing primer pLNCX primer (Common Sequencing Primers)
Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.
miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).
The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTRE-TIGHT miR-1 (aka:pTRE-m1d) was a gift from David Bartel (Addgene plasmid # 14896 ; http://n2t.net/addgene:14896 ; RRID:Addgene_14896)
For your References section:Prediction of mammalian microRNA targets. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Cell. 2003 Dec 26. 115(7):787-98. 10.1016/S0092-8674(03)01018-3 PubMed 14697198