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EML8543_pEML41003

Plasmid

#237864

Purpose

A suicide vector for Agrobacterium with kanamycin resistance.

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_ALFA_H2B

Plasmid

#244055

Purpose

anchoring mCherry-1x ALFAto the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-ALFA-HIST1H2BJ

Tags

mCherry-ALFA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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bp::PhiC31

Plasmid

#140505

Purpose

Encodes PhiC31 recombinase downstream of a minimal promoter element

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

PhiC31

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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bp::PhiC31

Plasmid

#140505

Purpose

Encodes PhiC31 recombinase downstream of a minimal promoter element

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

PhiC31

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-1α-Venus-Nup107-FRT

Plasmid

#247344

Purpose

Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

Nup107 (NUP107) (NUP107 Human)

Tags

Venus

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CAaNa::GFP

Plasmid

#140507

Purpose

Encodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

attP-Neomycin-PolyA-attB

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CAaNa::GFP

Plasmid

#140507

Purpose

Encodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

attP-Neomycin-PolyA-attB

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF3134

Plasmid

#144621

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TFE3 (TFE3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF3134

Plasmid

#144621

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TFE3 (TFE3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET29a-α3-E

Plasmid

#187935

Purpose

Express phage alpha3 lysis protein E in bacteria

Depositor

Yi Ma

Article

Ma et al Bioengineering (Basel). 2022 Sep 2;9(9):

Insert

Phage alpha 3 lysis protein E

Tags

Strep II

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/LCN2/Hygro] Vector

Plasmid

#236831

Purpose

Express LCN2 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

LCN2 (LCN2 Human)

Use

Luciferase

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Endo-InPGrp

Plasmid

#236003

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Endo-InPGrp (Cyth3 Rat, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pIAeBlueHPV16LR

Plasmid

#185894

Purpose

In vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)

Expression

Yeast

Mutation

None

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx1

Plasmid

#183653

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A24_STOP

Plasmid

#161146

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A24 (SLC25A24 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 phiC31

Plasmid

#68310

Purpose

In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

Depositor

Konrad Basler

Article

Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

Insert

phiC31

Use

Rna transcription

Promoter

CMV

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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