232,493 results

1. TFORF0886

   Plasmid

   #143446

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CUX2 (CUX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-hM4D(Gi)-mCherry (AAV5)

   Viral Prep

   #50479-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-GFAP-hM4D(Gi)-mCherry (#50479). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM4D(Gi)-mCherry plasmid DNA. GFAP-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   GFAP

   Tags

   mCherry

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

3. mCherry-CD9-10

   Plasmid

   #55013

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD9 (CD9 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CD9-10

   Plasmid

   #55013

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD9 (CD9 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1284

   Plasmid

   #143946

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SALL1 (SALL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. K9L mutant of H3K9me3 biosensor

   Plasmid

   #120807

   Purpose

   FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
   

   Expression

   Mammalian

   Mutation

   lysine 9 is mutated to leucine 9 on histone H3

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Antares2 c-myc

   Plasmid

   #100027

   Purpose

   A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   Antares2
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Antares2 c-myc

   Plasmid

   #100027

   Purpose

   A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   Antares2
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-EF1a-Archon1-KGC-EGFP-ER2

   Plasmid

   #108425

   Purpose

   Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS-DSCP

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS-DSCP

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-NFAT luciferase

    Plasmid

    #17870

    Purpose

    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    3x NFAT binding sequence (IL2 )
    

    Use

    Luciferase

    Tags

    IL2 promoter and Luciferase

    Expression

    Mammalian

    Promoter

    IL-2 promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-NFAT luciferase

    Plasmid

    #17870

    Purpose

    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    3x NFAT binding sequence (IL2 )
    

    Use

    Luciferase

    Tags

    IL2 promoter and Luciferase

    Expression

    Mammalian

    Promoter

    IL-2 promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tom20-V5-FKBP-AP_pLX304

    Plasmid

    #120914

    Purpose

    Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    Tom20-V5-FKBP-AP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-OTUB1 WT-pcDNA3.1

    Plasmid

    #118209

    Purpose

    Expresses FLAG-OTUB1 WT in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Red Glifon 3000

    Plasmid

    #163116

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 3000
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MEF2D-2A-neo

    Plasmid

    #190622

    Purpose

    Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

    Depositor

    Junwei Shi

    Article

    Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

    Insert

    MEF2D (MEF2D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-GFP(0)

    Plasmid

    #199166

    Purpose

    used to express a neutral GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(0)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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