|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71655||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 4751
- Total vector size (bp) 5662
Modifications to backboneBackbone has 600 bp homology arms for integration into SS9. The upstream (5') homology contains a PAM mutation to inactive SS9_gRNA cleavage.
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
SpeciesSynthetic; Cloning vector pGFPuv
Insert Size (bp)911
- Promoter T7A1
- Cloning method Gibson Cloning
- 5′ sequencing primer AGTGACAAAAATAAATTATTTATTTATCCAGAAAATGAATTGGAAAATC
- 3′ sequencing primer CACATACTTATTATGAATGATAAAATTCATTCAATTAATAACAC (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSS9 was a gift from Ryan Gill (Addgene plasmid # 71655 ; http://n2t.net/addgene:71655 ; RRID:Addgene_71655)
For your References section:Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, Mutalik VK, Arkin AP, Gill RT. ACS Synth Biol. 2016 Apr 22. 10.1021/acssynbio.5b00187 PubMed 27072506