|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85811||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3831
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
SpeciesStreptococcus pyogenes strain SF370
Insert Size (bp)4107
- Promoter pBAD
- Cloning method Gibson Cloning
- 5′ sequencing primer ccataagattagcggatcctacctgacgc
- 3′ sequencing primer ggtgggtatgtggtcgaaggctgg (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX2-Cas9 was a gift from Ryan Gill (Addgene plasmid # 85811 ; http://n2t.net/addgene:85811 ; RRID:Addgene_85811)