MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
(Plasmid #86457)

• Purpose: Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites
• Depositing Lab: Ralf Kuehn
• Publication: Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11.

Backbone

• Vector backbone: pBS
• Backbone size w/o insert (bp): 3125
• Total vector size (bp): 8767
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BFP
• Alt name: Blue fluorescent protein
• Insert Size (bp): 720
• GenBank ID: GQ221702.1 GQ221702.1
• Promoter: Pgk

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: M13 for
• 3′ sequencing primer: M13 rev

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry