Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.
Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kuhn R, Rajewsky K
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11. PubMed Article
Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kuhn R, Rajewsky K
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86457 | MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP | Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites |