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A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.
Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
Zebrafish. 2016 Sep 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74632p5E--3mnx1Can be used to drive expression specifically in zebrafish motor neurons
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75023p5E-mpeg1.1Can be used to drive expression in zebrafish microglia.
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75024p5E-gfapCan be used to drive expression in zebrafish astrocytes.
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75025p5E-elavl3Can be used to drive pan neuronal expression in zebrafish.
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75148pME-mTagBFPTagBFP (blue) fluorophore for use with 5’ promoters.
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75149pME-mTagBFP-CAAXMembrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.
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75153pME-mVenus-CAAXMembrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.
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75154pME-H2B-mCerulean3-P2A-mKOFP2-CAAXNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
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75155pME-mKOFP2-CAAXMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.
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75156pME-H2B-mCerulean3Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.
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75158pME-loxP-mTagBFP2-stop-pA-loxPFloxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.
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75174p3E-MCSMultiple cloning site for insertion of gene of interest for C-terminal fusions.
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75175p3E-mTagBFPTagBFP (blue) fluorophore with stop codon for C-terminal fusions
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75176p3E-mTagBFP-CAAXMembrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions
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75177p3E-H2B-mCerulean3-P2A-mKOFP2-CAAXNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.
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75179p3E-mCherry-NS-MCSmCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
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75180p3E-EGFP-NS-MCSEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
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75341pME-EGFP-NSMultisite gateway entry clone for adding N-terminal fusions of EGFP
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75342pME-TagBFP-NSMultisite gateway entry clone for adding N-terminal fusions of TagBFP
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75343pME-TagRFPt-NSMultisite gateway entry clone for adding N-terminal fusions of TagRFPt
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78250pTol2p2A--3mnx1:mTagBFP-NS-MCSCan be used to subclone in gene of interest into blue motor neuron expression vector.
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78251pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCSCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.
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