|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74632||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2642
- Total vector size (bp) 5507
Modifications to backbonen/a
Vector type5' entry vector for Multisite Gateway Three Fragment vector construction kit(Invitrogen)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemotor neuron and pancreas homeobox 1 promoter
Alt name-3mnx1 promoter, Hb9 promoter
SpeciesD. rerio (zebrafish)
Insert Size (bp)2864
Entrez Genemnx1 (a.k.a. hlxb9, zgc:112174)
- Promoter -3mnx1
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
Please note Addgene's quality control sequence found an 8bp gap when compared to the depositor's sequence. This gap is located in a T-rich region and does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p5E--3mnx1 was a gift from Nicholas Cole (Addgene plasmid # 74632 ; http://n2t.net/addgene:74632 ; RRID:Addgene_74632)
For your References section:A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880