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Addgene

p5E--3mnx1
(Plasmid #74632)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74632 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONRP2R-P3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2642
  • Total vector size (bp) 5507
  • Modifications to backbone
    n/a
  • Vector type
    5' entry vector for Multisite Gateway Three Fragment vector construction kit(Invitrogen)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    motor neuron and pancreas homeobox 1 promoter
  • Alt name
    -3mnx1 promoter, Hb9 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2864
  • Entrez Gene
    mnx1 (a.k.a. hlxb, hlxb9, zgc:112174)
  • Promoter -3mnx1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note Addgene's quality control sequence found an 8bp gap when compared to the depositor's sequence. This gap is located in a T-rich region and does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E--3mnx1 was a gift from Nicholas Cole (Addgene plasmid # 74632 ; http://n2t.net/addgene:74632 ; RRID:Addgene_74632)
  • For your References section:

    A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880