|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75153||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2565
- Total vector size (bp) 3345
Vector typeMiddle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)780
- Promoter n/a
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe mVenus-CAAX portion of this plasmid was ordered as a GeneArt™ Genestring™ fragment from ThermoFisher Scientific.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-mVenus-CAAX was a gift from Nicholas Cole (Addgene plasmid # 75153 ; http://n2t.net/addgene:75153 ; RRID:Addgene_75153)
For your References section:A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880