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pTol2p2A--3mnx1:mTagBFP-NS-MCS
(Plasmid #78250)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78250 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTol2pA2
  • Backbone manufacturer
    Chien Lab
  • Backbone size w/o insert (bp) 4143
  • Total vector size (bp) 7892
  • Vector type
    Zebrafish motor neuron expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    -3mnx1
  • Alt name
    mnx1, Hb9
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3742
  • Entrez Gene
    mnx1 (a.k.a. hlxb9, zgc:112174)
  • Promoter -3mnx1

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Tol2-F [5'-CAATCCTGCAGTGCTGAAAA-3']
  • 3′ sequencing primer EBV-rev [5'-GTGGTTTGTCCAAACTCATC-3']
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    -mTagBFP-NS-MCS

Resource Information

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2p2A--3mnx1:mTagBFP-NS-MCS was a gift from Nicholas Cole (Addgene plasmid # 78250 ; http://n2t.net/addgene:78250 ; RRID:Addgene_78250)
  • For your References section:

    A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880