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USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.
Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61741pRK5-FLAG-USP13express N-terminally FLAG tagged human USP13
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61742pRK5-FLAG-USP5express N-terminally FLAG tagged human USP5
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61743PRK5-FLAG-USP13/5-1express N-terminally FLAG tagged human chimera USP13/5-1
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61744PRK5-FLAG-USP13/5-2express N-terminally FLAG tagged human chimera USP13/5-2
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61745PRK5-FLAG-USP13/5-3express N-terminally FLAG tagged human chimera USP13/5-3
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61746PRK5-FLAG-USP13/5-4express N-terminally FLAG tagged human chimera USP13/5-4
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61747PRK5-FLAG-USP13/5-5express N-terminally FLAG tagged human chimera USP13/5-5
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61748PRK5-FLAG-USP13/5-6express N-terminally FLAG tagged human chimera USP13/5-6
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61749pet42c(+)-GST-USP13express N-terminally GST tagged human USP13
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61750pet42c(+)-GST-USP5express N-terminally GST tagged human USP5
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61751pCDNA-gp78-FLAG RING mutant C356G H361Aexpress C-terminally FLAG tagged human gp78
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61809pCMV-Ubl4A K48Rexpresses mutated Ubl4A with K48R, with a C-terminal FLAG tag
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61810pcDNA 5/FR/TO-Ubl4Aexpress C-terminal Ubl4A, for making the stable cell line
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61811pcDNA 5/FR/TO-Ubl4A K48Rexpress C-terminal Ubl4A K48R, for making the stable cell line
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61812px330-USP13to make USP13 Knockout cell line
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61833px330-gp78to make gp78 Knockout cell line
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61836PRK5-FLAG-Bag6express N-terminally FLAG tagged human Bag6
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61837PRK5-FLAG-Bag6ΔUBLexpress N-terminally FLAG tagged human Bag6 without UBL domain
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62370pcDNA-gp78-FLAGExpress C-terminally FLAG tagged gp78
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