USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.
Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61741 | pRK5-FLAG-USP13 | express N-terminally FLAG tagged human USP13 |
| 61742 | pRK5-FLAG-USP5 | express N-terminally FLAG tagged human USP5 |
| 61743 | PRK5-FLAG-USP13/5-1 | express N-terminally FLAG tagged human chimera USP13/5-1 |
| 61744 | PRK5-FLAG-USP13/5-2 | express N-terminally FLAG tagged human chimera USP13/5-2 |
| 61745 | PRK5-FLAG-USP13/5-3 | express N-terminally FLAG tagged human chimera USP13/5-3 |
| 61746 | PRK5-FLAG-USP13/5-4 | express N-terminally FLAG tagged human chimera USP13/5-4 |
| 61747 | PRK5-FLAG-USP13/5-5 | express N-terminally FLAG tagged human chimera USP13/5-5 |
| 61748 | PRK5-FLAG-USP13/5-6 | express N-terminally FLAG tagged human chimera USP13/5-6 |
| 61749 | pet42c(+)-GST-USP13 | express N-terminally GST tagged human USP13 |
| 61750 | pet42c(+)-GST-USP5 | express N-terminally GST tagged human USP5 |
| 61751 | pCDNA-gp78-FLAG RING mutant C356G H361A | express C-terminally FLAG tagged human gp78 |
| 61809 | pCMV-Ubl4A K48R | expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag |
| 61810 | pcDNA 5/FR/TO-Ubl4A | express C-terminal Ubl4A, for making the stable cell line |
| 61811 | pcDNA 5/FR/TO-Ubl4A K48R | express C-terminal Ubl4A K48R, for making the stable cell line |
| 61812 | px330-USP13 | to make USP13 Knockout cell line |
| 61833 | px330-gp78 | to make gp78 Knockout cell line |
| 61836 | PRK5-FLAG-Bag6 | express N-terminally FLAG tagged human Bag6 |
| 61837 | PRK5-FLAG-Bag6ΔUBL | express N-terminally FLAG tagged human Bag6 without UBL domain |
| 62370 | pcDNA-gp78-FLAG | Express C-terminally FLAG tagged gp78 |