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USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.

Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61741pRK5-FLAG-USP13express N-terminally FLAG tagged human USP13
61742pRK5-FLAG-USP5express N-terminally FLAG tagged human USP5
61743PRK5-FLAG-USP13/5-1express N-terminally FLAG tagged human chimera USP13/5-1
61744PRK5-FLAG-USP13/5-2express N-terminally FLAG tagged human chimera USP13/5-2
61745PRK5-FLAG-USP13/5-3express N-terminally FLAG tagged human chimera USP13/5-3
61746PRK5-FLAG-USP13/5-4express N-terminally FLAG tagged human chimera USP13/5-4
61747PRK5-FLAG-USP13/5-5express N-terminally FLAG tagged human chimera USP13/5-5
61748PRK5-FLAG-USP13/5-6express N-terminally FLAG tagged human chimera USP13/5-6
61749pet42c(+)-GST-USP13express N-terminally GST tagged human USP13
61750pet42c(+)-GST-USP5express N-terminally GST tagged human USP5
61751pCDNA-gp78-FLAG RING mutant C356G H361Aexpress C-terminally FLAG tagged human gp78
61809pCMV-Ubl4A K48Rexpresses mutated Ubl4A with K48R, with a C-terminal FLAG tag
61810pcDNA 5/FR/TO-Ubl4Aexpress C-terminal Ubl4A, for making the stable cell line
61811pcDNA 5/FR/TO-Ubl4A K48Rexpress C-terminal Ubl4A K48R, for making the stable cell line
61812px330-USP13to make USP13 Knockout cell line
61833px330-gp78to make gp78 Knockout cell line
61836PRK5-FLAG-Bag6express N-terminally FLAG tagged human Bag6
61837PRK5-FLAG-Bag6ΔUBLexpress N-terminally FLAG tagged human Bag6 without UBL domain
62370pcDNA-gp78-FLAGExpress C-terminally FLAG tagged gp78

Antibodies from Article