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(Plasmid #89105)


Item Catalog # Description Quantity Price (USD)
Plasmid 89105 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human glucocorticoid receptor
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    NR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Addgene sequencing results found a 4 AA insertion after G499 and an I747T mutation compared to NCBI reference sequence NP_000167.1. The depositor has confirmed that this construct produces functional GR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GR11 was a gift from Elizabeth Wilson (Addgene plasmid # 89105 ; ; RRID:Addgene_89105)
  • For your References section:

    Receptor accessory factor enhances specific DNA binding of androgen and glucocorticoid receptors. Kupfer SR, Marschke KB, Wilson EM, French FS. J Biol Chem. 1993 Aug 15;268(23):17519-27. PubMed 8349631