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Addgene

pk7-GR-GFP
(Plasmid #15534)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15534 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pk7-GFP
  • Backbone size w/o insert (bp) 5460
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glucocorticoid receptor
  • Alt name
    pk7-GR-GFP
  • Alt name
    pk-GR-GFP
  • Alt name
    GR-GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2333
  • Mutation
    The glucocorticoid receptor was cloned into the XbaI site in pk7-GFP such that the stop codon of GR was deleted and the coding sequence continued in frame with that of the GFP
  • GenBank ID
    NM_001018077
  • Entrez Gene
    NR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CAG GTC CAA CTG CAC CTC GGT TC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pk7-GR-GFP was a gift from Ian Macara (Addgene plasmid # 15534 ; http://n2t.net/addgene:15534 ; RRID:Addgene_15534)
  • For your References section:

    Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import. Carey KL, Richards SA, Lounsbury KM, Macara IG. J Cell Biol. 1996 Jun . 133(5):985-96. 10.1083/jcb.133.5.985 PubMed 8655589