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Ralf Kuehn Lab Plasmids


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Title Authors Publication
Ralf Kuehn TALEN plasmids (unpublished)
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P, Rajewsky K, Kuhn R BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kuhn R, Rajewsky K Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11.
Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector. Merkl C, Saalfrank A, Riesen N, Kuhn R, Pertek A, Eser S, Hardt MS, Kind A, Saur D, Wurst W, Iglesias A, Schnieke A PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31.
Kuehn -sgRNA plasmids (unpublished)
pSpCas9(BB)-2A-miRFP670 (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
45955pCAG-TALEN-A-pATALEN (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45961pCAG-TALEN-C-pATALEN (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45962pCAG-TALEN-G-pATALEN (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45963pCAG-TALEN-T-pATALEN (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45964pCMV-TALEN-Repbeta-GalactosidaseMammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pCMVbeta Add to Cart
45965pCAG-RabZFN-ARabZFN-A (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45966pCAG-RabZFN-BRabZFN-B (Synthetic)Mammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pBluescript Add to Cart
45967pCMV-Rab-Repbeta-GalactosidaseMammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pCMVbeta Add to Cart
45968pCMV-LucLuciferaseMammalian Expression Ralf Kuehn TALEN plasmids (unpublished) pGL3 basic Add to Cart
64215pAAVS1-TLR targeting vectortraffic light reporter (TLR) (Homo sapiens)Human targetingIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct AAVS1 SA-2A-puro-pA donor AAVS1 AAV Add to Cart
64216pU6-sgRosa26-1_CBh-Cas9-T2A-BFPCas9 (Other), sgRNA targeting ROSA26-1Mammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide pU6-(BbsI)_CBh-Cas9-T2A-BFP Add to Cart
64217pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)Cas9 (Other), hU6 promoter; BbsI sites for sgRNA, H1 promoter; BamHI site for shRNAMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A pU6-(BbsI)_CBh-Cas9-T2A-BFP Add to Cart
64218pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1BCas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP Add to Cart
64219pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1BCas9 (Other), sgRNA targeting ROSA26-1Mammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B Add to Cart
64220pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6Cas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP Add to Cart
64221pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1BCas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A pU6-(BbsI)_CBh-Cas9-T2A-mCherry Add to Cart
64222pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6Cas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-mCherry Add to Cart
64322pTLR repair vectorVenus (Other)Artificial targeting vectorIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Vector for repair of the traffic light reporter (TLR), providing the venus coding region Bluescript Add to Cart
64323pU6-(BbsI)_CBh-Cas9-T2A-BFPCas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide pX330 Add to Cart
64324pU6-(BbsI)_CBh-Cas9-T2A-mCherryCas9 (Other), sgRNA cassetteMammalian Expression, CRISPRIncreasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide pX330 Add to Cart
74281pR26 CAG/GFP DestRosa26 5-homology region (Mus musculus)Mouse TargetingEfficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning Bluescript Add to Cart
74282pR26 CAG/BFP DestRosa26 5-homology region (Mus musculus)Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning Bluescript Add to Cart
74283pR26 GFP DestRosa26 5-homology region (Mus musculus)Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning Bluescript Add to Cart
74284pR26 iBFP DestRosa26 5-homology region (Mus musculus)Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning Bluescript Add to Cart
74285pR26 CAG/GFP AscRosa26 5-homology region (Mus musculus)Mouse TargetingEfficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI Bluescript Add to Cart
74286pR26 CAG AsiSI/MluIRosa26 5-homology region (Mus musculus)Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4. Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui Bluescript Add to Cart
86457MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFPBFP (Other)Mammalian Expression, CRISPREfficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11. Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites pBS Add to Cart
86557pREPROII-attBOct4 (Mus musculus), cMyc (Mus musculus), Sox2 (Mus musculus), Kruppel-like factor 4 (Mus musculus)Mammalian Expression, Cre/LoxEfficient generation of rat induced pluripotent stem cells using a non-viral inducible vector. PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31. Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP Bluescript Pou5f1 NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4 Myc AU016757, Myc2, Niard, Nird, bHLHe39 Sox2 Sox-2, lcc, ysb Klf4 EZF, Gklf, Zie Add to Cart
86984pCAG-Trex2-bpAmouse Trex2 coding region (Mus musculus)Mammalian Expression Kuehn -sgRNA plasmids (unpublished) Expression of mouse Trex2 pBluescript(+) Trex2 Add to Cart
86985U6-(BbsI)sgRNA_CAG-Venus-bpAVenus (Other)Mammalian Expression Kuehn -sgRNA plasmids (unpublished) For cloning and expression of sgRNA together with Venus expression pBluescript(-) Add to Cart
86986pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpACas9-venus (Synthetic)Mammalian Expression Kuehn -sgRNA plasmids (unpublished) For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein Bluescript Add to Cart
86987pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFPCas9 (Other)Mammalian Expression Kuehn -sgRNA plasmids (unpublished) For cloning and expression of sgRNA together with expression of Cas9 and TagRFP Bluescript Add to Cart
91854pSpCas9(BB)-2A-miRFP670hSpCas9-2A-miRFP670 (Synthetic), BB-guide RNA (Synthetic)Mammalian Expression, CRISPR pSpCas9(BB)-2A-miRFP670 (unpublished) Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988 PX459 Add to Cart