PurposeContains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53750||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x membrane linker
- Promoter simian CMV IE94
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Cut with NotI and transcribe with SP6 for RNA.
CDS of mCherry was PCR amplified to add AgeI at N terminus and SnaBI at C-terminus. Put
this fragment in AgeI/SnaBI cut pCS-memb-cerulean (Addgene plasmid 53749).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS-memb-mCherry was a gift from Sean Megason (Addgene plasmid # 53750 ; http://n2t.net/addgene:53750 ; RRID:Addgene_53750)
For your References section:In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112