PurposeContains human Histone H2B fused to EGFP in the pCS2+ expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53744||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namehistone H2B
Alt namehistone cluster 2, H2be
SpeciesH. sapiens (human)
Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter simian CMV IE94
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB1 (destroyed during cloning)
- 3′ cloning site SnaB1 (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byH2B was obtained from Dr. Reinhard Koster.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cut with NotI and use SP6 for sense RNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS-H2B-EGFP was a gift from Sean Megason (Addgene plasmid # 53744 ; http://n2t.net/addgene:53744 ; RRID:Addgene_53744)
For your References section:In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112