PurposeContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53748||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehistone H2B
Alt namehistone cluster 2, H2be
SpeciesH. sapiens (human)
Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter simian CMV IE94
/ Fusion Protein
- Cerulean (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Use NotI/SP6 for sense RNA.
This plasmid was made using a triple ligation of the H2B from XhoI/AgeI of pCS-H2B-EGFP (Addgene plasmid 53744), the cerulean from AgeI/BsrG1 of pCeruleanC1 and pCS from BsrG1/XhoI of pCS-H2B-EGFP. This puts H2B-cerulean in the SnaBI site of pCS.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS-H2B-cerulean was a gift from Sean Megason (Addgene plasmid # 53748 ; http://n2t.net/addgene:53748 ; RRID:Addgene_53748)
For your References section:In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112