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pCS-H2B-cerulean
(Plasmid #53748)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53748 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone H2B
  • Alt name
    histone cluster 2, H2be
  • Species
    H. sapiens (human)
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • Cerulean (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H2B was obtained from Dr. Reinhard Koster.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Use NotI/SP6 for sense RNA.

This plasmid was made using a triple ligation of the H2B from XhoI/AgeI of pCS-H2B-EGFP (Addgene plasmid 53744), the cerulean from AgeI/BsrG1 of pCeruleanC1 and pCS from BsrG1/XhoI of pCS-H2B-EGFP. This puts H2B-cerulean in the SnaBI site of pCS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-H2B-cerulean was a gift from Sean Megason (Addgene plasmid # 53748 ; http://n2t.net/addgene:53748 ; RRID:Addgene_53748)
  • For your References section:

    In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112