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In toto imaging of embryogenesis with confocal time-lapse microscopy.
Megason SG
Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
53744pCS-H2B-EGFPContains human Histone H2B fused to EGFP in the pCS2+ expression vector
53745pCS-H2B-mRFP1Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector
53746pCS-H2B-EYFPcontains fusion of human histone H2B to EYFP in pCS2+ vector.
53748pCS-H2B-ceruleanContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.
53749pCS-membrane-ceruleanFPContains membrane localized cerulean fluorescent protein in pCS expression vector.
53750pCS-memb-mCherryContains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.
53752pCS-H2B-citrineContains fusion of human Histone H2B to citrine.

Antibodies from Article