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pCS-H2B-citrine
(Plasmid #53752)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53752 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone H2B
  • Alt name
    histone cluster 2, H2be
  • Species
    H. sapiens (human)
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • citrine (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H2B was obtained from Dr. Reinhard Koster.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created using the QuikChange protocol to perform site directed mutagenesis to make Q69M mutation to convert EYFP of pCS-H2B-EYFP (Addgene plasmid # 53746) into citrine.

Use NotI/SP6 for transcription.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-H2B-citrine was a gift from Sean Megason (Addgene plasmid # 53752 ; http://n2t.net/addgene:53752 ; RRID:Addgene_53752)
  • For your References section:

    In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112