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pLPC-Flag-PML-IV
(Plasmid #62804)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62804 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PML-IV
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1902
  • Entrez Gene
    PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
  • Tag / Fusion Protein
    • 3X-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer taggcgtgtacggtggga
  • 3′ sequencing primer acctgtaggtttggcaag
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid # 62804 ; http://n2t.net/addgene:62804 ; RRID:Addgene_62804)
  • For your References section:

    Regulation of E2Fs and senescence by PML nuclear bodies. Vernier M, Bourdeau V, Gaumont-Leclerc MF, Moiseeva O, Begin V, Saad F, Mes-Masson AM, Ferbeyre G. Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111. 10.1101/gad.1975111 PubMed 21205865