Purposeexpresses the isoform IV of PML (promyelocytic leukemia protein)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62804||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7480
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1902
Entrez GenePML (a.k.a. MYL, PP8675, RNF71, TRIM19)
/ Fusion Protein
- 3X-FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer taggcgtgtacggtggga
- 3′ sequencing primer acctgtaggtttggcaag (Common Sequencing Primers)
The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid # 62804 ; http://n2t.net/addgene:62804 ; RRID:Addgene_62804)
For your References section:Regulation of E2Fs and senescence by PML nuclear bodies. Vernier M, Bourdeau V, Gaumont-Leclerc MF, Moiseeva O, Begin V, Saad F, Mes-Masson AM, Ferbeyre G. Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111. 10.1101/gad.1975111 PubMed 21205865