|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50939||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4076
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namepromyelocytic leukemia
SpeciesH. sapiens (human)
Insert Size (bp)6022
MutationK65R, K160R, K490R (Sumo modification sites)
Entrez GenePML (a.k.a. MYL, PP8675, RNF71, TRIM19)
- Promoter SV40
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PML-3/pSG5 was a gift from Pier Pandolfi (Addgene plasmid # 50939 ; http://n2t.net/addgene:50939 ; RRID:Addgene_50939)
For your References section:Role of SUMO-1-modified PML in nuclear body formation. Zhong S, Muller S, Ronchetti S, Freemont PS, Dejean A, Pandolfi PP. Blood. 2000 May 1;95(9):2748-52. PubMed 10779416
Map uploaded by the depositor.