A bright and photostable photoconvertible fluorescent protein.
McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
Nat Methods. 2009 Feb . 6(2):131-3. PubMed Article
McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
Nat Methods. 2009 Feb . 6(2):131-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 20341 | pRSETa mEos2 | bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein) |
| 54510 | mEos2-C1 | Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581 |
| 54632 | mEos2-Display-5 | Localization: Cytoplasm, Excitation: 505 / 569, Emission: 516 / 581 |
| 54662 | mEos2-N1 | Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581 |
| 54750 | mEos2-Peroxisomes-2 | Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581 |
| 54809 | mEos2-Lifeact-7 | Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581 |
| 57340 | mEos2-Actin-C-18 | Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581 |
| 57344 | mEos2-Alpha-5-Integrin-12 | Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57350 | mEos2-Beta-Catenin-20 | Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57351 | mEos2-CAF1-C-10 | Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581 |
| 57352 | mEos2-Calnexin-N-14 | Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581 |
| 57353 | mEos2-Calreticulin-N-10 | Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581 |
| 57354 | mEos2-CathespinB-6 | Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581 |
| 57355 | mEos2-Caveolin-C-10 | Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57356 | mEos2-CD9-10 | Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57357 | mEos2-CD36-C-10 | Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57358 | mEos2-CD151-7 | Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57359 | mEos2-CENPA | Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581 |
| 57360 | mEos2-CENPB-N-22 | Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 516 / 581 |
| 57361 | mEos2-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 505 / 569, Emission: 516 / 581 |
| 57362 | mEos2-Coronin1B-C-10 | Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581 |
| 57363 | mEos2-C-Src-7 | Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 516 / 581 |
| 57364 | mEos2-Cx26-7 | Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57365 | mEos2-Cx43-7 | Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57367 | mEos2-Dectin1A-C-10 | Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57368 | mEos2-Dectin1A-N-10 | Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57370 | mEos2-Desmocollin1-N-18 | Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581 |
| 57371 | mEos2-EB1-C-18 | Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581 |
| 57372 | mEos2-EB3-7 | Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581 |
| 57373 | mEos2-ER-5 | Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581 |
| 57376 | mEos2-Fascin-C-10 | Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581 |
| 57377 | mEos2-Fibrillarin-7 | Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581 |
| 57378 | mEos2-FilaminA-C-14 | Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57379 | mEos2-FilaminA-N-9 | Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57382 | mEos2-Golgi-7 | Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581 |
| 57383 | mEos2-H1-10 | Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581 |
| 57384 | mEos2-H2B-6 | Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581 |
| 57386 | mEos2-Hec1-C-12 | Localization: Kinetochore, Excitation: 505 / 569, Emission: 516 / 581 |
| 57389 | mEos2-Integrin-Alpha2-N-18 | Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57390 | mEos2-Integrin-Beta1-N-18 | Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57391 | mEos2-Integrin-Beta3-N-18 | Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57392 | mEos2-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581 |
| 57395 | mEos2-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581 |
| 57396 | mEos2-LC-Myosin-N-7 | Localization: Light Chain Myosin, Excitation: 505 / 569, Emission: 516 / 581 |
| 57397 | mEos2-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581 |
| 57398 | mEos2-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 505 / 569, Emission: 516 / 581 |
| 57399 | mEos2-MAP4-C-10 | Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581 |
| 57401 | mEos2-Mito-7 | Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581 |
| 57409 | mEos2-Paxillin-22 | Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57410 | mEos2-PMP-N-10 | Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581 |
| 57432 | mEos2-Tubulin-C-18 | Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581 |
| 57434 | mEos2-VASP-N-10 | Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57435 | mEos2-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581 |
| 57438 | mEos2-Vinculin-14 | Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581 |
| 57701 | Dendra2-Actin-C-18 | Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573 |
| 57715 | Dendra2-EB3-7 | Localization: MT End Binding Protein, Excitation: 490 / 553, Emission: 507 / 573 |
| 57721 | Dendra2-Gamma-Tubulin-17 | Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573 |
| 57722 | Dendra2-Golgi-7 | Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573 |
| 57726 | Dendra2-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573 |
| 57728 | Dendra2-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573 |
| 57744 | Dendra2-VASP-5 | Localization: Actin/Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573 |
| 57745 | Dendra2-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573 |
| 57748 | Dendra2-Vinculin-23 | Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573 |
| 57750 | Dendra2-Zyxin-6 | Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573 |