167,318 results

1. TFORF2118

   Plasmid

   #141929

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBPL1 (TBPL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3_cAMPFIRE-M

   Plasmid

   #182280

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YES1 gRNA (BRDN0001148961)

   Plasmid

   #77967

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YES1 gRNA (BRDN0001145317)

   Plasmid

   #77965

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YES1 gRNA (BRDN0001487120)

   Plasmid

   #77966

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpCas9-CBE6c-V106W

   Plasmid

   #215826

   Purpose

   Express CBE6c V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-hSyn-nucGFP

    Plasmid

    #140190

    Purpose

    Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    GFP-NLS
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbA8k-RFP

    Plasmid

    #35273

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_IRGM_WT

    Plasmid

    #82134

    Purpose

    Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRGM (IRGM Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHM130

    Plasmid

    #225529

    Purpose

    Encodes destablized GFP under IBRE4 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    GFP
    BFP
    

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-PLXNB2

    Plasmid

    #86237

    Purpose

    Lentivirus for expression of human Plexin-B2

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    full length PLXNB2, CRISPR resistant

    Promoter

    human PGK promoter

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EMMA toolkit

    Plasmid Kit

    #1000000119

    Purpose

    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

    Depositor

    Yizhi Cai

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pCAS9-mCherry-Frame +0

    Plasmid

    #66939

    Purpose

    CRISPaint frame selector +0

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +0
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI-Neo HA-hSnoN

    Plasmid

    #10908

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    SnoN (SKIL Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only