Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs.
Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas DF
Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 10.1038/s41477-020-0670-y. PubMed Article
Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas DF
Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 10.1038/s41477-020-0670-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149275 | SPDK3876(TRV-RNA2-pPEBV-MCS) | Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs |
| 149276 | SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA) | Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs |
| 149277 | SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA) | Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs |
| 149278 | SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA) | Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs |
| 149279 | pEE083 | T-DNA encoding TRV2 complete genome with MCS |
| 149280 | pEE390 | T-DNA encoding TRV2 with gRNA targeting NbPDS3 |
| 149281 | pEE391 | T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3 |
| 149282 | pEE392 | T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3 |
| 149283 | pEE393 | T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3 |
| 149284 | pEE386 | T-DNA encoding TRV2 with gRNA targeting NbAG |
| 149285 | pEE387 | T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG |
| 149286 | pEE388 | T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG |
| 149287 | pEE389 | T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG |
| 149288 | pEE515 | T-DNA encoding TRV2 complete genome with MCS |
| 149289 | pEE385 | T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG |
| 149290 | pEE531 | T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2 |
| 149291 | pEE495 | T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2 |
| 149292 | pEE491 | T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2 |
| 199245 | SPDK3860 | Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene |