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RED
(Plasmid #139835)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 139835 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Life Technologies/Thermo Fisher
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 10922
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNase H1-EGFP-dCas9
  • Alt name
    RNase H1-EGFP-deadCas9
  • Species
    RNase H1 (H. sapiens), dCas9 (S. pyogenes)
  • Insert Size (bp)
    5844
  • Mutation
    dCas9 protein contains two mutations: D10A and H840A
  • GenBank ID
    RNase H1 (BT019670.1) Cas9 (AAK33936.1)
  • Entrez Gene
    RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
  • Promoter tet-inducible CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RED was a gift from Karim Mekhail (Addgene plasmid # 139835 ; http://n2t.net/addgene:139835 ; RRID:Addgene_139835)
  • For your References section:

    Nucleolar RNA polymerase II drives ribosome biogenesis. Abraham KJ, Khosraviani N, Chan JNY, Gorthi A, Samman A, Zhao DY, Wang M, Bokros M, Vidya E, Ostrowski LA, Oshidari R, Pietrobon V, Patel PS, Algouneh A, Singhania R, Liu Y, Yerlici VT, De Carvalho DD, Ohh M, Dickson BC, Hakem R, Greenblatt JF, Lee S, Bishop AJR, Mekhail K. Nature. 2020 Sep;585(7824):298-302. doi: 10.1038/s41586-020-2497-0. Epub 2020 Jul 15. 10.1038/s41586-020-2497-0 PubMed 32669707