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Daniel Zenklusen Lab Plasmids


The Daniel Zenklusen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23.
The nuclear basket mediates perinuclear mRNA scanning in budding yeast. Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger M, Zenklusen D J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
45162pDZ415 (24MS2SL loxP-Kan-loxP)24xMS2 Stem Loop Cassette (Synthetic)Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. pKAN Add to Cart
45163pDZ416 (24PP7SL loxP-Kan-loxP)24xPP7 Stem Loop Cassette (Synthetic)Cre/LoxSingle-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. pKAN Add to Cart
45929pDZ274 (pLEU MET25pro MCP-2x-yeGFP)MCP-2x-yeGFPYeast ExpressionSingle-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. pLEU Add to Cart
45930pSH100 (YCplac33 MET25pro MCP-mCherry)MCP-mCherryYeast ExpressionSingle-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. YCplac33 Add to Cart
45931pDZ513 (pHIS MET25pro PP7-PS-yeGFP)PCP-PSYeast ExpressionSingle-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. pHIS Add to Cart
72230pDZ514 pURA MET25p PP7-PS-2x-yeGFPPP7-PS-2x-yeGFP (Synthetic)Yeast ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter pURA Add to Cart
72233pDZ529 pTRP MET25p PP7-PS-2x-yeGFPPP7-PS-2x-yeGFP (Synthetic)Yeast ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter pTRP Add to Cart
72234pDZ536 pURA ADE3p PP7-PS-2x-yeGFPPP7-PS-2x-yeGFP (Synthetic)Yeast ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter pURA Add to Cart
72236pDZ585 pKAN 2x-mCherry2x-mCherry (Other)Bacterial ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry pKAN Add to Cart
72237pDZ617 pKAN 12xPP7 V412xPP7 V4 (Synthetic)Bacterial Expression, Cre/LoxThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest pKAN Add to Cart
72240pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>GLT1 GAL1p 24xPP7 V3 (Synthetic)Bacterial ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging) pHIS Add to Cart
73172pDZ584 pKAN 1x-mCherry1x-mCherry (Synthetic)UnspecifiedThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry pKAN Add to Cart
73173pDZ645 pKAN 1x-mCherry-12xPP7 V41x-mCherry (Synthetic)Mammalian Expression, Yeast ExpressionThe nuclear basket mediates perinuclear mRNA scanning in budding yeast. J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070. Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest pKAN Add to Cart