164,744 results

1. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

   Plasmid

   #221235

   Purpose

   Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC70206

   Plasmid

   #209467

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM60 FLAG-RAB35 Q67L

   Plasmid

   #107105

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynap.iGABASnFR (AAV1)

   Viral Prep

   #112159-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.hSynap.iGABASnFR (#112159). In addition to the viral particles, you will also receive purified pAAV.hSynap.iGABASnFR plasmid DNA. Synapsin-driven iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Promoter

   Syn

   Tags

   None

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pMpGWB105

   Plasmid

   #68559

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p404ADH1

   Plasmid

   #15970

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-eYFP (AAV Retrograde)

   Viral Prep

   #104055-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-CAG-eYFP (#104055). In addition to the viral particles, you will also receive purified pAAV-CAG-eYFP plasmid DNA. CAG-driven eYFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   CAG

   Tags

   EYFP

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PA-GFP-α-Tubulin

    Plasmid

    #213900

    Purpose

    Imaging of Tubulin using Photoactivatable GFP

    Depositor

    Lukas Kapitein

    Insert

    PA-GFP-α-Tubulin
    

    Use

    Tags

    Photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFLAG-GATA2

    Plasmid

    #1418

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

    Insert

    GATA-2 (GATA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSNAPf-Cox8A Control Plasmid

    Plasmid

    #101129

    Purpose

    Expression of SNAP-Tagged Cox8A in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Cox8A (COX8A Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MSCV-N E4orf1

    Plasmid

    #38063

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    E4 orf1
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmRFP-N1 human cofilin S3A

    Plasmid

    #50857

    Purpose

    expresses constituvely active cofilin fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S3A constitutively active

    Promoter

    CMV

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSUC01

    Plasmid

    #177204

    Purpose

    chromogenic reporter for gene knockout in Streptomyces

    Depositor

    Wenjun Guan

    Article

    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

    Insert

    SaindC
    

    Use

    Synthetic Biology

    Tags

    NONE

    Expression

    Bacterial

    Mutation

    NONE

    Promoter

    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDe-CAS9

    Plasmid

    #61433

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUBI4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4-p52

    Plasmid

    #23289

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 human cofilin S3A

    Plasmid

    #50860

    Purpose

    expresses constituvely active cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3A constitutively active

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-kdrl:mCherry-caax

    Plasmid

    #194287

    Purpose

    Expression plasmid for zebrafish or Danionella cerebrum

    Depositor

    Pui-Ying Lam

    Article

    Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

    Insert

    kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
    

    Use

    Zebrafish or danionella cerebrum expression

    Tags

    mCherryCAAX from the Tol2kit (Kwan et al., 2007)

    Expression

    Mutation

    Promoter

    kdrl(flk1)

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-3c-rhVTN_FL

    Plasmid

    #30223

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin without deletions (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No deletions

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge