181,096 results
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Plasmid#131477PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmyc-active WRN (WRN Human)
Tags5x mycExpressionMammalianAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-mCherry-IRES-Flpo (AAV1)
Viral prep#55634-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID
UseDrosophila expressionAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDB302
Plasmid#113673Purposeexpresses Cth SUMO protease in Escherichia coliDepositorInsertCth SUMO protease
Tags10-HisExpressionBacterialMutationdeleted amino acids 1-934PromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCT310
Plasmid#111140PurposeBacterial expression of 6His-dCas9-3XFlagDepositorInsertdCas9
Tags3X Flag and 6X HisExpressionBacterialMutationD10A - H840APromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralExpressionMammalianPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6 asyn WT
Plasmid#107425PurposeMammalian expression of human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein (SNCA Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSyn.iGluSnFr.WPRE.SV40 (AAV1)
Viral prep#98929-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFr.WPRE.SV40 (#98929). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFr.WPRE.SV40 plasmid DNA. Synapsin-driven, iGluSnFr glutamate sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceFeb. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40
Plasmid#105540PurposeAAV expression of EGFP-Cre from hSyn promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertEGFP-Cre
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-TFEBS211A-MYC (#805)
Plasmid#99957Purposemammalian expressionDepositorAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP SNAP23
Plasmid#101914PurposeExpresses SNAP23 in mammalian cellsDepositorAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
Plasmid#90003PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPapi
Plasmid#96921Purposeexpression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MoClo Pichia toolkit
Plasmid kit#1000000108PurposeA set of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit (#1000000061) for protein expression and secretion in Pichia pastoris.DepositorApplicationCloning and Synthetic BiologyVector typeYeast ExpressionCloning typeGolden Gate (MoClo)Available sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-RB1
Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…ExpressionMammalianPromoterTight TRE promoter and U6Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIpA102-G-HLA-A2_GFP
Plasmid#85162Purposefor mRNA expression of HLA.A2-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHLA.A2 (HLA-A Human)
UseMrna expressionTagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85006PurposeFor Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-ires-tTA
ExpressionMammalianAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by