Purposefor mRNA expression of HLA.A2-EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85162||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression ; mRNA expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Entrez GeneHLA-A (a.k.a. HLAA)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byIt was cloned from the cDNA of an healthy donor and sequence confirmed as HLA-A*0201
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCI-A102 constructs were opened with MfeI and used as a template for small-scale mRNA synthesis
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIpA102-G-HLA-A2_GFP was a gift from Tone Fredsvik Gregers & Sébastien Walchli (Addgene plasmid # 85162 ; http://n2t.net/addgene:85162 ; RRID:Addgene_85162)
For your References section:Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation. Walchli S, Kumari S, Fallang LE, Sand KM, Yang W, Landsverk OJ, Bakke O, Olweus J, Gregers TF. Eur J Immunol. 2014 Mar;44(3):774-84. doi: 10.1002/eji.201343671. Epub 2013 Dec 27. 10.1002/eji.201343671 PubMed 24293164