WRN helicase is a synthetic lethal target in microsatellite unstable cancers.
Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, Gonzalez A, McPartlan JS, Li T, Zhang Y, Bin Liu J, Lazaro JB, Gu P, Piett CG, Apffel A, Ali SO, Deasy R, Keskula P, Ng RWS, Roberts EA, Reznichenko E, Leung L, Alimova M, Schenone M, Islam M, Maruvka YE, Liu Y, Roper J, Raghavan S, Giannakis M, Tseng YY, Nagel ZD, D'Andrea A, Root DE, Boehm JS, Getz G, Chang S, Golub TR, Tsherniak A, Vazquez F, Bass AJ
Nature. 2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 10.1038/s41586-019-1102-x. PubMed Article
Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, Gonzalez A, McPartlan JS, Li T, Zhang Y, Bin Liu J, Lazaro JB, Gu P, Piett CG, Apffel A, Ali SO, Deasy R, Keskula P, Ng RWS, Roberts EA, Reznichenko E, Leung L, Alimova M, Schenone M, Islam M, Maruvka YE, Liu Y, Roper J, Raghavan S, Giannakis M, Tseng YY, Nagel ZD, D'Andrea A, Root DE, Boehm JS, Getz G, Chang S, Golub TR, Tsherniak A, Vazquez F, Bass AJ
Nature. 2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 10.1038/s41586-019-1102-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125767 | pXPR_BRD003-sgCh2-2 | constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) |
| 125768 | pXPR_BRD003-sgCh2-4 | constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) |
| 125769 | pXPR_BRD003-sgPOLR2D | constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control) |
| 125770 | pXPR_BRD003-sgMYC | constitutive expression of a guide RNA targeting human MYC (CRISPR positive control) |
| 125771 | pXPR_BRD003-sgWRN1 | constitutive expression of a guide RNA targeting human WRN |
| 125772 | pXPR_BRD003-sgWRN2 | constitutive expression of a guide RNA targeting human WRN |
| 125773 | pXPR_BRD003-sgWRN3 | constitutive expression of a guide RNA targeting human WRN |
| 125774 | pXPR_BRD003-sgWRN-EIJ | constitutive expression of a guide RNA targeting an exon-intron junction of human WRN |
| 125775 | pXPR_BRD051-sgCh2-2 | constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9 |
| 125776 | pXPR_BRD051-sgMLH1-1 | constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9 |
| 125777 | pXPR_BRD051-sgMLH1-2 | constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9 |
| 125778 | pLKO.1-puro-shRFP | constitutive expression of a short-hairpin RNA targeting RFP |
| 125779 | pLKO.1-puro-shPSMD2 | constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control) |
| 125780 | pLKO.1-puro-shRPS6 | constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control) |
| 125781 | pLKO.1-puro-shWRN1 | constitutive expression of a short-hairpin RNA targeting human WRN |
| 125782 | pLKO.1-puro-shWRN2 | constitutive expression of a short-hairpin RNA targeting human WRN |
| 125783 | pRSITEP-puro-shWRN1 | DOX-inducible expression of a short-hairpin RNA targeting human WRN |
| 125784 | pRSITEP-puro-shWRN1-C911 | a seed-matched control for pRSITEP-puro-shWRN1 |
| 125785 | pRSITEP-puro-shWRN2 | DOX-inducible expression of a short-hairpin RNA targeting human WRN |
| 125786 | pRSITEP-puro-shWRN2-C911 | a seed-matched control for pRSITEP-puro-shWRN2 |
| 125788 | pLX209-neo-active WRN | constitutive, ectopic expression of human active WRN |
| 125789 | pLX209-neo-WRN E84A | constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A) |
| 125790 | pLX209-neo-WRN K577M | constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M) |
| 125791 | pLX209-neo-WRN E84A K577M | constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN |