spNW30
(Plasmid #173485)

• Purpose: Expresses spNW30 in Bl21
• Depositing Lab: Huan Bao
• Depositing Organization: UF Scripps Biomedical Research
  Located in United States of America
• Publication: Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi: 10.1038/s41467-021-25737-7.

Backbone

• Vector backbone: pet28a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: spNW30
• Species: Synthetic
• Insert Size (bp): -5
• Tag / Fusion Protein:
  • Histag (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to be prone to dimerization. Dimerization often does not impact plasmid function but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.