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Addgene

pRSETa_ mEos4b
(Plasmid #51073)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51073 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSETa
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3584
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEos4b
  • Species
    Synthetic
  • Insert Size (bp)
    684
  • GenBank ID
    KJ126790
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GGGATGGGGATCCATGGTGAGTGCGATTAAGCCAGACAT
  • 3′ sequencing primer GGGCCCCGAATTCTTATCGTCTGGCATTGTCAGGCAATC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETa_ mEos4b was a gift from Loren Looger (Addgene plasmid # 51073 ; http://n2t.net/addgene:51073 ; RRID:Addgene_51073)
  • For your References section:

    Fixation-resistant photoactivatable fluorescent proteins for CLEM. Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL. Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225. 10.1038/nmeth.3225 PubMed 25581799