pcDNA3 tdAPX W41F
PurposeTandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40863||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6996
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert nametandem dimer pea APX W41F
Insert Size (bp)1608
- Promoter CMV
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMVf
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
This plasmid is for expression of tandem dimer pea APX W41F in mammalian cells under the CMV promoter. In this construct, tdAPX is not fused to a signal peptide, so its expression is whole-cell. The insert is not mammalian codon optimized.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 tdAPX W41F was a gift from Alice Ting (Addgene plasmid # 40863 ; http://n2t.net/addgene:40863 ; RRID:Addgene_40863)
For your References section:Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203