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pcDNA3 tdAPX W41F
(Plasmid #40863)


Item Catalog # Description Quantity Price (USD)
Plasmid 40863 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
    tandem dimer pea APX W41F
  • Species
  • Insert Size (bp)
  • Mutation
    W41F mutation
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVf
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is for expression of tandem dimer pea APX W41F in mammalian cells under the CMV promoter. In this construct, tdAPX is not fused to a signal peptide, so its expression is whole-cell. The insert is not mammalian codon optimized.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 tdAPX W41F was a gift from Alice Ting (Addgene plasmid # 40863 ; ; RRID:Addgene_40863)
  • For your References section:

    Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203