|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42608||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6198
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert namesoybean APX W41F (dimeric) -NES
Insert Size (bp)810
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMVf (Common Sequencing Primers)
This plasmid is for expression of soybean APX W41F (dimeric) in mammalian cells under the CMV promoter. In this construct, APX has a C-terminal nuclear export signal (NES), with amino acid sequence LQLPPLERLTLD.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-APX(W41F)-NES was a gift from Alice Ting (Addgene plasmid # 42608 ; http://n2t.net/addgene:42608 ; RRID:Addgene_42608)
For your References section:Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Science. 2013 Jan 31. 10.1126/science.1230593 PubMed 23371551