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pCAG APX W41F dimeric (mito matrix)
(Plasmid #40307)


Item Catalog # Description Quantity Price (USD)
Plasmid 40307 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
    pea APX W41F (dimeric)
  • Alt name
    CAG promoter, expression in mitochondrial matrix
  • Species
  • Insert Size (bp)
  • Mutation
    W41F mutation.
  • Promoter CAG
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is for expression of pea APX W41F (dimeric) in the mitochondrial matrix under the CAG promoter. In this construct, APX has a mitochondrial localization peptide (MLATRVFSLVGKRAISTSVCVRAH) fused to its N-terminus. The APX gene is mammalian codon optimized.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG APX W41F dimeric (mito matrix) was a gift from Alice Ting (Addgene plasmid # 40307 ; ; RRID:Addgene_40307)
  • For your References section:

    Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203