pCAG APX W41F dimeric (mito matrix)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40307||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5651
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert namepea APX W41F (dimeric)
Alt nameCAG promoter, expression in mitochondrial matrix
Insert Size (bp)747
- Promoter CAG
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
This plasmid is for expression of pea APX W41F (dimeric) in the mitochondrial matrix under the CAG promoter. In this construct, APX has a mitochondrial localization peptide (MLATRVFSLVGKRAISTSVCVRAH) fused to its N-terminus. The APX gene is mammalian codon optimized.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG APX W41F dimeric (mito matrix) was a gift from Alice Ting (Addgene plasmid # 40307 ; http://n2t.net/addgene:40307 ; RRID:Addgene_40307)
For your References section:Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203