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Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy.
Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY
Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
40306pUC57 APEXA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
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40307pCAG APX W41F dimeric (mito matrix)
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40863pcDNA3 tdAPX W41FTandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).
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44439pcDNA3 Connexin43-APEX
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44440pcDNA3 Connexin43-GFP-APEX
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