pBabe-puro-IMS-RP
(Plasmid #24535)

• Depositing Lab: Peter Sorger
• Publication: Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Backbone

• Vector backbone: pBabe-puro
• Backbone manufacturer: Bob Weinberg, Addgene plasmid 1764
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Diablo homolog
• Alt name: DIABLO, SMAC
• Alt name: mitochondrial intermembrane space reporter protein (IMS-RP)
• Species: H. sapiens (human)
• Insert Size (bp): 867
• Mutation: Deleted residues 56-239
• Entrez Gene: DIABLO (a.k.a. DFNA64, SMAC)
• Tag / Fusion Protein:
  • mRFP1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IMS-RP, a reporter for MOMP that localizes to the mitochondrial intermembrane space, was constructed by PCR of bp 1-147 of Smac-1, ligation to the 5′ end of monomeric RFP-1, and cloning into pBabe-Puro. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information