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pBabe-puro-IMS-RP
(Plasmid #24535)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24535 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Bob Weinberg, Addgene plasmid 1764
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Diablo homolog
  • Alt name
    DIABLO, SMAC
  • Alt name
    mitochondrial intermembrane space reporter protein (IMS-RP)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    867
  • Mutation
    Deleted residues 56-239
  • Entrez Gene
    DIABLO (a.k.a. DFNA64, SMAC)
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

IMS-RP, a reporter for MOMP that localizes to the mitochondrial intermembrane space, was constructed by PCR of bp 1-147 of Smac-1, ligation to the 5′ end of monomeric RFP-1, and cloning into pBabe-Puro. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro-IMS-RP was a gift from Peter Sorger (Addgene plasmid # 24535 ; http://n2t.net/addgene:24535 ; RRID:Addgene_24535)
  • For your References section:

    Modeling a snap-action, variable-delay switch controlling extrinsic cell death. Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK. PLoS Biol. 2008 Dec 2. 6(12):2831-52. 10.1371/journal.pbio.0060299 PubMed 19053173